IntelliFlow™Platelet Stabilizer · Human Cat. No. ZK202605 · 50 vials / pack · Screw cap
FOR RESEARCH USE ONLY · NOT FOR USE IN DIAGNOSTIC PROCEDURES

Platelet Stabilizer

Provides a validated pre-analytical solution for flow cytometric analysis of human platelet activation. The stabilization reagent halts further ex vivo platelet activation immediately after specimen transfer, preserving CD62P (P-selectin) and other surface glycoprotein epitopes in their state at the time of collection — addressing the sample-handling delays that published studies have shown to cause artifactual platelet activation in flow cytometry.

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Product Features

Core capabilities

  • Improved state stabilityInhibits platelet activation in vitro and maintains the platelet status present at the time of specimen collection.
  • Extended sample validityStable for 7 days at room temperature (22–28°C), enabling domestic multi-site collection and transport without cold chain. Also stable at 2–8°C for applications requiring refrigerated or international shipment.
  • Simple transfer workflowCollect a specimen into a standard 3.2% sodium citrate tube, then use a calibrated pipette to add 0.5 mL of specimen to the Platelet Stabilizer. Gently invert 5 to 8 times to mix. The stabilization solution is supplied ready to use — no reagent preparation.
  • Multi-scenario applicationsSuitable for research experiments, multi-center studies, and settings requiring extended storage or long-distance transport.
Scope of Application

Research settings

Research Laboratories

Sample preparation for platelet flow cytometry at research institutions and academic laboratories.

Research Platform

Platelet function research, drug screening, and sample processing for related research studies.

Multi-Center Projects

Standardized processing of samples requiring extended storage or transport across multiple collection sites.

Direction for Use

Workflow

1

Collect & Transfer

Collect a specimen into a standard 3.2% sodium citrate vacuum collection tube using standard venipuncture technique for research specimen collection. Within 5 minutes of collection, use a calibrated pipette to add 0.5 mL of specimen from the citrate collection tube to the Platelet Stabilizer.

2

Mix

Gently invert the vial 5 to 8 times to ensure thorough mixing of the specimen with the stabilization solution. Do not shake.

3

Store / Analyze

Store at room temperature (22–28°C) or refrigerated (2–8°C). Samples remain stable for up to 7 days under either condition. No cold chain required for shipping at room temperature; refrigerated conditions (2–8°C) suitable for applications requiring refrigerated or international shipment.

Important information: For Research Use Only. Not for use in diagnostic procedures. Do not use for direct venipuncture — specimen transfer only. Wear appropriate PPE and follow institutional biosafety regulations. The specimen-to-additive ratio is critical — do not overfill or underfill. Do not reuse the vial. Performance may vary due to individual sample differences and transit conditions.
Performance Characteristics · Fig. 1

CD62P stability over 7 days

Internal validation study. Venous specimens from healthy donors collected into 3.2% sodium citrate tubes, transferred into Platelet Stabilizer vials, and monitored by multicolor flow cytometry at 22–28°C and 2–8°C. n = 10 healthy donors. SD <2%.

Fig 1A
Fig. 1A — 3.2% Citrate-Anticoagulated Venous Blood (Control). Progressive ex vivo activation reaching 41.4% (22–28°C) and 65.1% (2–8°C) by Day 7.
Fig 1B
Fig. 1B — With Platelet Stabilizer. CD62P⁺ remains ≤1.5% through Day 7 at both temperature conditions. SD <2%.
Fig 1C
Fig. 1C — ADP-Activated Platelets, Citrate Tube (Control). Signal drifts to 94.5% (22–28°C) and 89.5% (2–8°C) by Day 7.
Fig 1D
Fig. 1D — ADP-Activated Platelets with Platelet Stabilizer. Activated signal stable at ~71–74% through Day 7 under both conditions.
Performance Characteristics · Fig. 2

CD62P scatter plots — representative sample

Multicolor flow cytometry. CD61/SSC gating followed by CD62P-PE vs CD61-APC analysis. Select a figure to view. Click thumbnails to switch between panels.

Fig. 2B — 3.2% Citrate + Platelet Stabilizer

CD62P remains below 1.1% through Day 7 at both temperatures. The platelet cloud stays entirely in the CD62P-negative quadrant — indistinguishable from the baseline reading.

0.78%
0h baseline
1.08%
Day 7 · 4°C
0.98%
Day 7 · 25°C
Fig. 2B (3.2% Citrate + Platelet Stabilizer) — panel a — CD61/SSC Gate · CD61+ = 6.25%
a — CD61/SSC Gate · CD61+ = 6.25%
Fig. 2B (3.2% Citrate + Platelet Stabilizer) — panel b — 0h · CD62P⁺ = 0.78%
b — 0h · CD62P⁺ = 0.78%
Fig. 2B (3.2% Citrate + Platelet Stabilizer) — panel c — Day 7 / 4°C · CD62P⁺ = 1.08%
c — Day 7 / 4°C · CD62P⁺ = 1.08%
Fig. 2B (3.2% Citrate + Platelet Stabilizer) — panel d — Day 7 / 25°C · CD62P⁺ = 0.98%
d — Day 7 / 25°C · CD62P⁺ = 0.98%

Fig. 2A — 3.2% Citrate-Anticoagulated Venous Specimen (Control)

Progressive ex vivo activation clearly visible. By Day 7, the CD62P-positive cloud has expanded dramatically — the sample no longer reflects the donor's in vivo platelet state.

3.25%
0h baseline
56.07%
Day 7 · 4°C
31.54%
Day 7 · 25°C
Fig. 2A (3.2% Citrate-Anticoagulated Venous Specimen (Control)) — panel a — CD61/SSC Gate · CD61+ = 5.15%
a — CD61/SSC Gate · CD61+ = 5.15%
Fig. 2A (3.2% Citrate-Anticoagulated Venous Specimen (Control)) — panel b — 0h · CD62P⁺ = 3.25%
b — 0h · CD62P⁺ = 3.25%
Fig. 2A (3.2% Citrate-Anticoagulated Venous Specimen (Control)) — panel c — Day 7 / 4°C · CD62P⁺ = 56.07%
c — Day 7 / 4°C · CD62P⁺ = 56.07%
Fig. 2A (3.2% Citrate-Anticoagulated Venous Specimen (Control)) — panel d — Day 7 / 25°C · CD62P⁺ = 31.54%
d — Day 7 / 25°C · CD62P⁺ = 31.54%

Fig. 2D — ADP-Activated Platelets + Platelet Stabilizer

The Platelet Stabilizer locks in the activation state present at time of transfer. ADP-activated signal stable at ~72% through Day 7 at both temperatures — no drift.

72.32%
0h baseline
71.66%
Day 7 · 4°C
72.36%
Day 7 · 25°C
Fig. 2D (ADP-Activated Platelets + Platelet Stabilizer) — panel a — CD61/SSC Gate · CD61+ = 2.45%
a — CD61/SSC Gate · CD61+ = 2.45%
Fig. 2D (ADP-Activated Platelets + Platelet Stabilizer) — panel b — 0h · CD62P⁺ = 72.32%
b — 0h · CD62P⁺ = 72.32%
Fig. 2D (ADP-Activated Platelets + Platelet Stabilizer) — panel c — Day 7 / 4°C · CD62P⁺ = 71.66%
c — Day 7 / 4°C · CD62P⁺ = 71.66%
Fig. 2D (ADP-Activated Platelets + Platelet Stabilizer) — panel d — Day 7 / 25°C · CD62P⁺ = 72.36%
d — Day 7 / 25°C · CD62P⁺ = 72.36%

Fig. 2C — ADP-Activated Platelets, Citrate Tube (Control)

ADP-activated platelets in standard citrate continue to drift upward — from 81.9% at baseline to 93.71% at Day 7 / 4°C. The activation state is not preserved.

81.90%
0h baseline
93.71%
Day 7 · 4°C
91.93%
Day 7 · 25°C
Fig. 2C (ADP-Activated Platelets, Citrate Tube (Control)) — panel a — CD61/SSC Gate · CD61+ = 3.34%
a — CD61/SSC Gate · CD61+ = 3.34%
Fig. 2C (ADP-Activated Platelets, Citrate Tube (Control)) — panel b — 0h · CD62P⁺ = 81.90%
b — 0h · CD62P⁺ = 81.90%
Fig. 2C (ADP-Activated Platelets, Citrate Tube (Control)) — panel c — Day 7 / 4°C · CD62P⁺ = 93.71%
c — Day 7 / 4°C · CD62P⁺ = 93.71%
Fig. 2C (ADP-Activated Platelets, Citrate Tube (Control)) — panel d — Day 7 / 25°C · CD62P⁺ = 91.93%
d — Day 7 / 25°C · CD62P⁺ = 91.93%
Limitations
  • For Research Use Only. Not for use in diagnostic procedures.
  • Performance data established using samples from healthy donors. Performance in samples from subjects with underlying platelet abnormalities, active coagulopathy, or anticoagulant therapy has not been independently evaluated in this study.
  • Designed to preserve platelets' basal activation status, protecting against the artifactual activation that begins once platelets leave the body. If agonist stimulation is required, agonist must be added to citrate-anticoagulated specimen prior to transfer into the Platelet Stabilizer.
  • Specimen must be transferred within 5 minutes of specimen collection.
Publications

Selected literature on platelet flow cytometry
pre-analytical standardization

The following publications highlight the scientific rationale for standardized platelet sample handling.

CYTOMETRY PART B · 2025

Signal without noise: Practical antibody titration for platelet flow cytometry

Spurgeon BEJ et al.

Key insight: Standardizing sample handling under final assay conditions is critical for reproducibility — artifact introduced before analysis cannot be removed during data processing.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS · 2021

Consensus recommendations on flow cytometry for the assessment of inherited and acquired disorders of platelet number and function

Frelinger AL et al. — ISTH SSC

Key insight: 11 experts from 10 countries reached uniform agreement on instrument standardization, but 25.9% of pre-analytical variable statements were rated "uncertain" — pointing directly to the need for standardized preservation solutions.
ARTERIOSCLEROSIS, THROMBOSIS, AND VASCULAR BIOLOGY · 2024

High-Dimensional Single-Cell Mass Cytometry Demonstrates Differential Platelet Functional Phenotypes in Infants With Congenital Heart Disease

Gu SX et al.

Key insight: High-parameter platelet phenotyping depends critically on sample handling quality — pre-analytical degradation limits the ability to distinguish true biological variation from artifact.

These publications are cited as background literature relevant to the pre-analytical challenges addressed by the Platelet Stabilizer. IntelliFlow TECH LLC is not affiliated with the authors.

Frequently Asked Questions

Common questions

Must the specimen be transferred within 5 minutes?

Yes. Transfer from the citrate tube to the Platelet Stabilizer should occur within 5 minutes of collection to minimize ex vivo platelet activation before stabilization.

How should filled vials be shipped?

Filled vials can be shipped at ambient temperature (22–28°C) for up to 7 days, or at 2–8°C for refrigerated or international shipment. Ship filled vials according to institutional policies, carrier requirements, and applicable regulations based on the specimen risk assessment. Use leak-proof secondary containers and absorbent material.

Can I use any flow cytometer?

Yes. The Platelet Stabilizer is compatible with all major flow cytometer platforms and antibody vendors including BD Biosciences and Beckman Coulter. No special instrument settings are required.

Specifications
Product namePlatelet Stabilizer
Catalog numberZK202605
FormatScrew neck vial, 8-425 thread (ND8) · Clear Type I Class A borosilicate glass · screw cap with PTFE-lined septum
Transfer volume0.5 mL specimen · specimen-to-additive ratio 1:1
Dimensions12 mm OD × 32 mm height
Shelf life (unopened)24 months at 22–28°C · Do not freeze · Protect from direct sunlight
Post-transfer stability7 days at 22–28°C (room temperature) or 2–8°C (refrigerated)
Package size50 vials per pack
CompatibilityAll major flow cytometers · BD Biosciences · Beckman Coulter · all major antibody vendors
Regulatory status (US)For Research Use Only · Not for use in diagnostic procedures
LabelingEnglish labeling · English Instructions for Use (IFU)