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Selected literature on sample stability and pre-analytical standardization for platelet and leukocyte flow cytometry in human and veterinary research.
Platelet Flow Cytometry — Human
Cytometry Part B · 2025
Signal without noise: Practical antibody titration for platelet flow cytometry
Spurgeon BEJ et al.
Key insight: Standardizing sample handling under final assay conditions is critical for reproducibility — artifact introduced before analysis cannot be removed during data processing.
Journal of Thrombosis and Haemostasis · 2021
Consensus recommendations on flow cytometry for the assessment of inherited and acquired disorders of platelet number and function
Frelinger AL et al. — ISTH SSC
Key insight: 11 experts from 10 countries reached uniform agreement on instrument standardization, but 25.9% of pre-analytical variable statements were rated "uncertain" — pointing directly to the need for standardized preservation solutions.
Arteriosclerosis, Thrombosis, and Vascular Biology · 2024
High-Dimensional Single-Cell Mass Cytometry Demonstrates Differential Platelet Functional Phenotypes in Infants With Congenital Heart Disease
Gu SX et al.
Key insight: High-parameter platelet phenotyping depends critically on sample handling quality — pre-analytical degradation limits the ability to distinguish true biological variation from artifact.
Leukocyte Immunophenotyping — Human
Pathology · 2024
Effects of blood sample storage time, temperature, anti-coagulants and blood stabiliser on lymphocyte phenotyping
Lao Y et al. — SA Pathology / University of Adelaide
Key insight: Storage time — but not temperature — altered several lymphocyte subset percentages in whole blood over 24-48 hours; using a chemical blood stabilization reagent reduced the range of subsets affected, supporting stabilization as a way to preserve accurate results during transport delays.
Transplantation Research · 2013
Standardization of whole blood immune phenotype monitoring for clinical trials: panels and methods from the ONE study
Streitz M et al. — the ONE Study Consortium
Key insight: A multi-center consortium developed standardized whole-blood immunophenotyping panels and methods specifically to make lymphocyte subset data comparable across clinical trial sites — standardized sample handling matters as much as standardized panels for multi-center immune monitoring.
Indian Journal of Clinical Biochemistry · 2004
Enumeration of lymphocyte subsets using flow cytometry: Effect of storage before and after staining in a developing country setting
Jalla S et al.
Key insight: In field-based studies requiring transport from a remote collection site to a central laboratory, both delayed staining and delayed analysis altered lymphocyte subset percentages compared to same-day processing — the same remote-collection challenge that motivates standardized sample stabilization.
Platelet Flow Cytometry — Veterinary
Veterinary Clinical Pathology · 2023
Extended sample storage for platelet function testing in healthy dogs
Dickinson M et al. — Ontario Veterinary College, University of Guelph
Key insight: Delayed platelet function testing in dogs is feasible, but expected value ranges shift with storage time compared to fresh samples — underscoring why time-stable, standardized sample handling matters before analysis.
Journal of Veterinary Emergency and Critical Care · 2026
Assessment of Platelet Storage Lesions, Viability, and Function in Canine Platelet Concentrate Units Stored at 4°C for 14 Days
Farrell KS et al. — UC Davis / North Carolina State University
Key insight: Flow cytometric P-selectin and phosphatidylserine expression in canine platelets varied with storage time and agonist exposure — demonstrating that platelet activation state is a moving target unless handling is standardized.
Journal of Veterinary Internal Medicine · 2018
A Remote Assay for Measuring Canine Platelet Activation and the Inhibitory Effects of Antiplatelet Agents
Dunning M et al. — University of Nottingham
Key insight: Delayed flow cytometric measurement of canine platelet P-selectin, performed well after collection, reliably detected antiplatelet drug effects — supporting remote, stabilized platelet activation assays as a viable approach in veterinary antiplatelet-response research.
Leukocyte Immunophenotyping — Veterinary
Veterinary Clinical Pathology · 2014
Stability of immunophenotypic lymphoid markers in fixed canine peripheral blood for flow cytometric analysis
Cian F et al. — University of Cambridge
Key insight: Cyto-Chex BCT preserved several canine lymphocyte markers over storage, but CD3/CD45 and fluorescence intensity changed over time; stabilized samples can extend analysis windows, but marker-specific limitations must be considered.
Frontiers in Veterinary Science · 2024
Adapting the SMART tube technology for flow cytometry in feline full blood samples
Zwicklbauer K et al. — Ludwig-Maximilians-Universität München
Key insight: A fixative-based stabilization protocol adapted for feline whole blood allowed quantification of T-helper cells, cytotoxic T-cells, B-cells, monocytes, and neutrophils long after collection — demonstrating that standardized sample stabilization extends the practical window for feline immunophenotyping well beyond same-day analysis.
BMC Veterinary Research · 2020
Stability of canine and feline cerebrospinal fluid samples regarding total cell count and cell populations stored in TransFix/EDTA CSF sample storage tubes
Meier L et al. — University of Veterinary Medicine Hannover
Key insight: TransFix/EDTA CSF storage tubes supported extended flow cytometric analysis of lymphocytes and granulocytes, while monocyte detection and routine cytology still required caution or fresh samples.
Cited as background literature. IntelliFlow TECH LLC is not affiliated with the authors.